Figure 4

Comparison of the impact of anti-CD3/CD28 beads and soluble anti-CD3 plus irradiated MNCs on expansion by purified naive (A) and memory (B) CD8 cells. Significant differences in the phenotype of expanded naïve T cells, but not in memory cells, were noted. Panel C collates the results of 3 experiments quantitating changes in CD45RA, CCR7, CD27, and CD28 surface antigen (expressed as geometric mean channel fluorescence) when naïve and memoryCD8 cells were expanded using beads (squares) or anti-CD3/MNCs(circles) for 14 days.