Each mRNA expression profiles during 14 days at ICU. Changes in the circulating mRNA expression profile during the clinical course (post-operative days [POD] 0-14) in ICU. Relative ratio of mRNA expression compared with β-actin mRNA in serum is depicted as the longitudinal axis. We show the change in mRNA expression level for PDGFA, MUC1, PBEF1/NAMPT, TGF-β1, TNF-α, MMP9, EGR1, HMGB1, and VWF. IL-6 data and CRP data are provided in Figure 3 and Additional file 1, respectively. HMGB1 and EGR1 responded to surgery and being upregulated at POD 0. PDGFA, MUC1, and TNF-α peaked at POD 3. TGF-β1 and VWF started being upregulated from POD 3. PBEF1/NAMPT and MMP9 started being upregulated from POD 5. All genes examined in this study were upregulated at equal or greater levels than the level of β-actin mRNA during the 14 days of ICU stay.