Figure 5

Endogenous MxA expression in PDAC cell lines and resistance to viral infection. A) MxA expression in PDAC cell lines by Western Blot analysis. B) Citotopathic effect of Adenovirus wt on MxA+ (orange) versus MxA- (blu) PDAC cell lines. The vertical arrow indicates increased viral concentration, from 10⁶, 10⁷, 10⁸ DNA particles of Ad5. C) Number of viral particles measured by real time PCR after Adeno5 wt infection in MxA+ and MxA- PDAC cell lines (Ad5 DNA replication efficiency). Normalised to the Ad5 DNA amount present in Panc2 at 4^th dilution considered as 1 Correlation of MxA expression with Adeno5 infection efficiency. MxA positive (HPAFI, CFPAC, PSN1, top) and MxA negative (GER, PT45, Panc1, bottom) cells were infected with 1.36 pfu/cell, 13.6 pfu/cell and 136 pfu/cell of Ad5-CMV-GFP vector. D) FACS analysis profile of different PDAC cell lines after 2 days of Adeno5-CMV-GFP infection (13.6 pfu/cell). E) Luminescence analysis for the permissivity of MxA+ and MxA- to the adeno associated infection, data are shown as relative luciferase units (RLU).