Validation of miRNA array data using quantitative real-time polymerase chain reaction (qPCR) assay. A. Relative expression patterns for the let-7 miRNA that were quantitated by qPCR. Relative expression levels (y-axis) in umbilical cord blood were defined as a level of one for comparison. B. Confirmation of miR-96, miR-29c, miR-429 up-regulated expression in adult cells. C. Relative expression patterns of the GATA-1 regulated miRNA, miR-451 and miR-144, and hematopoietic tissue-specific microRNA, miR-142. Umbilical cord blood (open bars), adult blood (closed bars), (* p < 0.05), (** p < 0.01). Note differences in y-axis scales between the three panels.