Activation of human B cells by the agonist CD40 antibody CP-870,893 and augmentation with simultaneous toll-like receptor 9 stimulation

Carpenter, Erica L; Mick, Rosemarie; Rüter, Jens; Vonderheide, Robert H

doi:10.1186/1479-5876-7-93

Table 1 B cell activation marker expression in response to stimulation

From: Activation of human B cells by the agonist CD40 antibody CP-870,893 and augmentation with simultaneous toll-like receptor 9 stimulation

	Negative control stimulation		CP-870,893 (CP)		CpG ODN 2006 (CpG)		CP-870,893 plus CpG ODN 2006		Linear mixed effects model p value*
	Mean	SE	Mean	SE	Mean	SE	Mean	SE	CP v. neg	CpG v. neg	CP v. CpG	CP+CpG v. CP	CP+CpG v. CpG
Total CD19+ ^†
CD40 MFI	1928	92	3867	265	8828	738	10308	776	<0.001	<0.001	<0.001	<0.001	0.004
MHC I MFI	10623	591	23221	2098	27165	2026	40067	3481	<0.001	<0.001	0.001	<0.001	<0.001
MHC II MFI	30642	4979	82839	4675	83856	4703	108161	5250	<0.001	<0.001	0.91	0.009	0.01
%CD86+	15.7	3.3	58.6	5.3	73.8	4.4	82.6	3.4	<0.001	<0.001	0.05	0.003	0.34
%CD70+	7.7	2.6	33.5	4.8	39.2	6.1	51.2	5.9	<0.001	<0.001	0.25	<0.001	0.01
CD19+ CD27+ ^‡
CD40 MFI	2293	75	5625	524	13225	879	13951	680	<0.001	<0.001	<0.001	<0.001	0.56
MHC I MFI	15661	1650	30927	2008	37825	2687	53246	4910	<0.001	<0.001	0.002	<0.001	<0.001
MHC II MFI	33254	3509	98699	5195	97553	4251	121932	6400	<0.001	<0.001	0.89	0.007	0.02
%CD86+	27.3	2.7	64.5	5.0	74.0	3.5	83.5	2.5	<0.001	<0.001	0.10	0.002	0.17
%CD70+	18.8	1.7	56.2	1.9	62.3	2.1	74.4	2.6	<0.001	<0.001	0.02	<0.001	0.001
CD19+ CD27negative ^‡
CD40 MFI	2195	108	4413	368	10417	1003	10622	804	<0.001	<0.001	<0.001	<0.001	0.88
MHC I MFI	7770	759	17726	1499	20993	2504	26777	4267	<0.001	<0.001	0.010	<0.001	0.14
MHC II MFI	40924	2749	90576	3333	86918	3847	96397	6585	<0.001	<0.001	0.60	0.47	0.22
%CD86+	13.2	1.6	61.1	5.1	75.8	4.9	82.8	3.5	<0.001	<0.001	<0.001	<0.001	0.13
%CD70+	5.0	0.6	30.3	3.3	34.8	3.8	41.3	5.1	<0.001	<0.001	0.009	<0.001	0.26

*Bold indicates p < 0.05
^†n = 8 normal donors ^‡n = 7 normal donors

Back to article page

ISSN: 1479-5876

Contact us

Submission enquiries: Access here and click Contact Us
General enquiries: info@biomedcentral.com

Journal of Translational Medicine

Contact us