Figure 1

Dendrimer 3a-G1 selectively inhibits CD4⁺ T cell proliferation among IL-2 cultured PBMCs during the first week of culture. a) Among PBMCs, NK and CD4⁺ T cells are the two major cell populations which spontaneously proliferate in response to IL-2 during the first week of culture. 3a-G1 not only enhances the proliferation of NK cells but it also affects the capacity of the CD4⁺ T cell population to proliferate. b) Average NK cell proliferation increased 29.4% ± 12.1% while CD4⁺ T cell proliferation decreased 66.1% ± 7.03% in 3a-G1 treated cultures compared to those containing only IL-2 (Day 7, n = 4). c) Impaired proliferation of CD4⁺ T cells in the presence of 3a-G1 is not rescued by higher IL-2 concentration after a week of culture. Results representative of two independent experiments performed on two individual donors. d) CD8⁺ T cell proliferation was induced adding anti-CD3/CD28 coated beads on IL-2 cultured PBMCs. The percentages indicated are expressed after gating on the relevant CD4⁺ or CD8⁺ T cell population. Addition of 3a-G1 in these conditions affected CD4⁺ as well as CD8⁺ T cell proliferation.