Figure 3

Treatment with ApoG2 induces alterations in the expression of c-Myc, p21, and cyclins. (A) The effect of ApoG2 on the expression of c-Myc. CNE-2 cells were incubated with 10 μ M ApoG2 for 24 to 72 h, and cell lysates were analyzed using immunoblotting. (B) The effect of ApoG2 on the expression of molecules downstream from c-Myc. After treatment with ApoG2, CNE-2 cell lysates were analyzed using immunoblotting with anti-p21, -cyclin D1, -cyclin E, and -p53 antibodies. (C) The effect of ApoG2 on cell cycle-regulatory molecules in HONE-1 cells. Cells were treated with 10 μ M ApoG2 for 24 to 72 h, and cell lysates were analyzed using immunoblotting.