Figure 3

Relative luciferase activity induced by transfection of human cell lines with Luc-H19 plasmid and the reduction of luciferase activity in human ovary primary culture from patient #1 and in human ovarian carcinoma cell lines due to co-transfection with the DTA-H19 vector. A. Relative luciferase activity, in OV-CAR, SKOV-3, TOV-112D and ES-2 human cell lines induced by transfection with Luc -H19 plasmid. Each cell line was transfected with 2 μg of Luc -H19 or the LucSV40 plasmid. The values represent the luciferase activity of the H19 promoter relative to the activity of the control vector LucSV40. B. The killing potential of the DTA-H19 vector in OVCAR-3 (blue), SKOV-3 (pink), TOV-112D (green), and ES-2 (orange) was measured as a reduction of LucSV40 activity. Cells were cotransfected with 2 μg LucSV40, and the indicated concentrations of DTA-H19 or LucSV40 alone. C. The killing potential of the DTA-H19 vector in human primary culture (blue) compared with SKOV-3 (pink) was measured as a reduction of Luciferase activity. Cells were transfected with 3 μg of LucSV40 alone, or cotransfected with 3 μg LucSV40 and the indicated concentrations of DTA-H19. Transfection experiments were stopped after 48 hours and luciferase activity was assessed. The activity of the luciferase in the LucSV40 transfected cells was compared to the luciferase activity in the cotransfected cells.