Figure 1

The level of H19 transcript in RNA isolated from cells of ascites fluid of different patients determined by RT-PCR or by ISH. A. H19 transcripts in the isolated ascites cells determined by ISH analysis. A positive stained cell is marked by a black arrow. B. The level of CA-125 in cells isolated from ascites fluid determined by IHC analysis (× 40 magnification). Black arrows mark the strong positive stains of cells expressing CA-125. C. The H19 transcript in RNA extracted from ascites cells determined by RT-PCR analysis. "M" 100-bp molecular weight marker. Line 1 – patient #1, Line 2 – patient # 2, Line 3 – patient # 3 and Line 4 – negative control. D. RT – PCR and ISH analysis of ascites cells from different patients. The RT-PCR results are expressed as positive (+) or negative (-). The ISH results are expressed as the number of moderate to strongly H19 positive samples. The intensity of hybridization signal was indicated as (+1) for weak, (+2) for moderate and (+3) for strong signals. The quantity of the staining was referred to (+1) up to one third of the cells, (+2) one to two thirds of the cells and (+3) more than two thirds of cells (I-indicates the intensity of the signal, Q-indicates the quantity of signal). Some samples could not be analyzed due to lack of material.