Development of a microarray platform for FFPET profiling: application to the classification of human tumors

Table 2 Probe content for FFPET 44 k v1.0 development array

OSD Name	# of Probes	OSD Parameter
OSD 0	3,996	Current default OSD
OSD 1	3,938	Current default OSD with increased weighting towards 3' end
OSD 2	4,003	Current default OSD weighted towards 100 nt from 3' end
OSD 3*	29,931	Tile at 30 nt up to 400 nt from 3' end
OSD 4	4,003	Tiled house keeping probes to 1000 nt from 3' end

Array represents approximately 4,000 unique genes with probes for OSD0 – OSD3. *OSD 3 was tiled, but any probes obviously failing standard oligonucleotide selection criteria for repeat sequences, bad base composition, and cross-hybridization were removed.

ISSN: 1479-5876