Figure 3
From: KSP inhibitor ARRY-520 as a substitute for Paclitaxel in Type I ovarian cancer cells
ARRY-520 induces apoptosis independent of the mitochondrial pathway. (a) Type II EOC cells were treated with 3 μM ARRY-520 for 12 and 24 hours, stained with JC-1 dye as described in the Materials and Methods section, and mitochondrial integrity was analyzed using Flow cytometry. (b) Graphical representation of the percentage of polarized and depolarized cells. Note that ARRY-520 does not induce mitochondrial depolarization. Results shown are obtained with CP70 cells. Similar results were observed with other cells tested.