Figure 1

Detection of classical MHC class I components in medulloblastoma samples. A) RNA was isolated from frozen tissue sections of individual medulloblastoma patients. Indicated are the specificities of the primers used and the size of each amplification product. Negative control (H₂O) contained no cDNA template. B) Representative sections showing staining with the heavy chain-specific monoclonal antibody HC-10 (×40 magnification). Tonsil tissue sections processed identically except for the absence (negative control) or presence (positive control) of primary HC-10 monoclonal antibody. Examples of medulloblastoma sections graded as 0, 1, 2 and 3. C) Representative sections showing staining with the CD45-specific monoclonal antibody (×40 magnification). Tonsil tissue sections processed identically except for the absence (a) or presence (b) of primary monoclonal antibody. Examples of medulloblastoma sections graded as 0, 1, 2 and 3.