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Table 3 Chemomodulation of phenotypic maturation of human DCs in vitro

From: Chemomodulation of human dendritic cell function by antineoplastic agents in low noncytotoxic concentrations

Marker HLA-DR CD83 CD80 CD86 CD40 CD1a
Agent       
Vinblastine, 1 nM 25.0 ± 4.5 72.7 ± 10.1* 16.5 ± 13.1 2.9 ± 3.5 46.1 ± 3.1* 16.7 ± 0.9
Vincristine, 1 nM 10.7 ± 0.7 25.9 ± 4.9 1.6 ± 0.2 27.0 ± 22.0 52.7 ± 2.9* 19.4 ± 0.3
Paclitaxel, 5 nM 0.5 ± 3.1 30.2 ± 5.7* 5.4 ± 27.5 6.9 ± 3.2 29.3 ± 3.4* 6.0 ± 2.3
5-aza-2-deoxycytidine, 5 nM 29.1 ± 12.2 8.1 ± 4.2 50.2 ± 3.2* 2.4 ± 6.4 33.4 ± 6.9* 10.8 ± 4.3
Methotrexate, 5 nM 3.6 ± 2.2 2.1 ± 1.8 6.5 ± 3.6 6.2 ± 0.8 51.0 ± 6.5* 35.9 ± 5.7*
Mitomycin C, 50 nM 4.2 ± 2.7 25.0 ± 12.8 12.0 ± 22.2 3.4 ± 0.9 24.9 ± 12.3 32.1 ± 5.8*
Doxorubicin, 10 nM 4.7 ± 0.3 38.8 ± 4.3* 4.24 ± 5.9 3.1 ± 2.0 14.3 ± 6.8 5.3 ± 7.1
  1. The results in Table 3, calculated from MFI values, are expressed as the percentage of MFI increase in drug-treated DCs in comparison to MFI in untreated DCs. Increase in any marker expression of greater than 30% was considered to be biologically significant and was analyzed for statistical significance of changes. Data represent the mean ± SEM from 3 independent experiments utilizing cells from 3 different healthy donors. *, p < 0.05 (ANOVA, N = 3).