Chemomodulation of human dendritic cell function by antineoplastic agents in low noncytotoxic concentrations

Table 3 Chemomodulation of phenotypic maturation of human DCs in vitro

Marker	HLA-DR	CD83	CD80	CD86	CD40	CD1a
Agent
Vinblastine, 1 nM	25.0 ± 4.5	72.7 ± 10.1*	16.5 ± 13.1	2.9 ± 3.5	46.1 ± 3.1*	16.7 ± 0.9
Vincristine, 1 nM	10.7 ± 0.7	25.9 ± 4.9	1.6 ± 0.2	27.0 ± 22.0	52.7 ± 2.9*	19.4 ± 0.3
Paclitaxel, 5 nM	0.5 ± 3.1	30.2 ± 5.7*	5.4 ± 27.5	6.9 ± 3.2	29.3 ± 3.4*	6.0 ± 2.3
5-aza-2-deoxycytidine, 5 nM	29.1 ± 12.2	8.1 ± 4.2	50.2 ± 3.2*	2.4 ± 6.4	33.4 ± 6.9*	10.8 ± 4.3
Methotrexate, 5 nM	3.6 ± 2.2	2.1 ± 1.8	6.5 ± 3.6	6.2 ± 0.8	51.0 ± 6.5*	35.9 ± 5.7*
Mitomycin C, 50 nM	4.2 ± 2.7	25.0 ± 12.8	12.0 ± 22.2	3.4 ± 0.9	24.9 ± 12.3	32.1 ± 5.8*
Doxorubicin, 10 nM	4.7 ± 0.3	38.8 ± 4.3*	4.24 ± 5.9	3.1 ± 2.0	14.3 ± 6.8	5.3 ± 7.1

The results in Table 3, calculated from MFI values, are expressed as the percentage of MFI increase in drug-treated DCs in comparison to MFI in untreated DCs. Increase in any marker expression of greater than 30% was considered to be biologically significant and was analyzed for statistical significance of changes. Data represent the mean ± SEM from 3 independent experiments utilizing cells from 3 different healthy donors. *, p < 0.05 (ANOVA, N = 3).

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