Skip to main content

Table 2 Sensitivity of human DCs to the cytotoxic effects of antineoplastic chemotherapeutic agents in vitro

From: Chemomodulation of human dendritic cell function by antineoplastic agents in low noncytotoxic concentrations

Chemotherapeutic agent
(concentration, nM)
Apoptosis of DCs
(% ± SEM)
vinblastine (50) 3.2 ± 0.9
vinblastine (10) 1.1 ± 1.3
vinblastine (1) 0.9 ± 0.3
vinblastine(0.1) -0.6 ± 0.3
vincristine (50) 6.5 ± 2.1
vincristine (10) 3.3 ± 1.9
vincristine (1) 0.5 ± 0.6
vincristine (0.1) -0.6 ± 0.9
paclitaxel (25) 4.9 ± 2.3
paclitaxel (5) 2.2 ± 0.7
paclitaxel (1) 2.2 ± 0.4
paclitaxel (0.1) 0.1 ± 0.3
5-aza-2deoxycitidine (25) 7.4 ± 3.3
5-aza-2deoxycitidine (5) 0.8 ± 0.8
methotrexate (25) 3.9 ± 1.1
methotrexate (5) 0.8 ± 0.6
methotrexate (1) 0.3 ± 0.4
mitomycin C (25) 1.3 ± 1.4
mitomycin C (5) -0.9 ± 0.4
doxorubicin (100) 5.5 ± 0.9
doxorubicin (25) 3.4 ± 0.3
doxorubicin (5) 0.6 ± 1.8
  1. Analysis of DC survival was carried out by flow cytometry after the staining with FITC-Annexin V and propidium iodide. DCs were treated with the cytotoxic agents for 48 h and analyzed by FACScan after staining. The background staining of control non-treated DC value was subtracted from experimental results. The results are express as the mean percentage of Annexin+PI- cells ± SEM of 3 independent assays. Student's t test was applied to compare the results of the treatment with different drug concentrations with control non-treated DC values in order to determine Effective Concentration (EC), i.e. the highest concentration of a chemotherapeutic agent that does not induce apoptosis in DCs.