Figure 2

Comparison of the fold change difference in expression between NOSE and TOV samples for the Ziplex and Affymetrix platforms. A: The log₂ fold change between the NOSE and TOV samples (mean NOSE signal intensity/mean TOV signal intensity) was calculated for the expression values of all 93 probes and plotted. Linear regression was performed resulting in the following model: log₂ Affymetrix NOSE/TOV = 0.180098 + 1.0251794 log₂ Ziplex NOSE/TOV with a Pearson's correlation coefficient (R) of 0.93. Probes that were not significant (p > 0.001 based on a Welch Rank Sum test) on either platform are indicated in grey, probes significant (p < 0.001 based on a Welch Rank Sum test) on both platforms are indicated in black, on only the Ziplex platform are indicated in blue and on only the Affymetrix platform in green. B: Bland-Altman plots for expression values of all probes. Values determined to be outliers are indicated in the mean-difference (of the log₂ fold change values) plot. A difference in log₂ fold change of 0 is indicated by a solid black line. The upper and lower 95% limits of agreement for the difference in log₂ fold change are indicated by red dashed lines, and arrows on the right hand side. Expression values that fall outside of these lines are considered outliers and are identified by their gene name.