Retinal pigment epithelial cells secrete neurotrophic factors and synthesize dopamine: possible contribution to therapeutic effects of RPE cell transplantation in Parkinson's disease

Table 2 DA and HVA in RPE cells extract and DA release after potassium treatment

	DA	HVA
Cells extract (ng/mg protein)	29.13 ± 4.11	267.89 ± 16.10
Release after potassium treatment	None	None

RPE cells were homogenated and centrifuged. The supernatant was examined by HPLC for DA and its metabolic. To determine the DA release, RPE cells were depolarized with high potassium (56 mM K⁺) and the buffer was subjected to HPLC assay.

ISSN: 1479-5876