Multilineage differentiation in vitro. Panel 1) Myogenic differentiation. K represents Kaleidoscope (BioRad, molecular marker), nC represents normal control of human skeletal muscle, T represents htMSCs control, Tm represents hFTs cells induced for myogenic differentiation. 1A) Dystrophin expression in muscle control and in the induced hFTs cells. 1B) Skeletal myosin expression in muscle control and in the induced hFTs cells. 1C) Myosin band observed in the muscle control and in the induced hFTs cells by Ponceau S membrane dyeing. 1D) IF assay, indicating dystrophin expression (in green fluorescence) in myotubes differentiated from hFTs cells, where nucleuses were colored with DAPI (blue fluorescence) (400×). Panel 2) Osteogenic, chondrogenic and adipogenic differentiation of hFTs cells. 2A) Control hFTs cells (630×). 2B) Osteogenic differentiation (200×). 2C) Chondrogenic differentiation (100×). 2D) Adipogenic differentiation (630×) (Microscope Zeiss Axiovert 200).