Figure 5From: HLA-A*0201-restricted CTL epitope of a novel osteosarcoma antigen, papillomavirus binding factorCytotoxic activity of CTL clones derived from CTL 5A9. Five CTL clones were established from CTL 5A9. Left panels indicate tetramer staining of CTL clones. CD8+ cells were gated. X-axis and Y-axis indicate the fluorescence intensity of control tetramer-FITC and A2/PBF A2.2 tetramer-PE, respectively. Middle panels indicate CTL-mediated cytotoxicity against T2 cells with or without PBF A2.2 peptide-pulsation. Right panels indicate CTL-mediated cytotoxicity against allogeneic osteosarcoma cell lines.Back to article page