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Figure 2 | Journal of Translational Medicine

Figure 2

From: HLA-A*0201-restricted CTL epitope of a novel osteosarcoma antigen, papillomavirus binding factor

Figure 2

Tetramer-based detection of PBF A2.2-specific T cells. CD8+ T cells (5.4 × 106) collected from Patient 4 were seeded into 36 wells at the concentration of 1.5 × 105 per well and cultured with peptide PBF A2.2 and cytokines. On day 21, tetramer analysis was performed. This analysis showed that 2 of 36 wells were positive, containing 0.03% and 0.39% tetramer-positive cells, respectively (A). The remaining 34 wells were negative with 0.00% reactivity. Here, 12 of 34 tetramer-negative wells are shown (B). Each of the 2 positive wells contained at least 1 CTL precursor, indicating that there were at least 2 CTL precursors in a total of 5.4 × 106 CD8+ cells. The frequency was calculated as 2/5.4 × 106 = 3.7 × 10-7.

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