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Figure 4 | Journal of Translational Medicine

Figure 4

From: Synthetic lethal RNAi screening identifies sensitizing targets for gemcitabine therapy in pancreatic cancer

Figure 4

Kinetic analysis of CHK1 siRNA induced sensitization of gemcitabine response. MIA PaCa-2 cells were transfected with either CHK1 siRNA or non-silencing siRNA and at 24 hours post transfection, cells were treated with either vehicle or 10 nM gemcitabine. Growth was assessed by impedance measurements at 1-hour intervals and cell index was plotted as a function of time. (A) Treatment of cells with non-silencing siRNA and either vehicle or gemcitabine showed a slight decrease in cell growth by gemcitabine. (B) Pretreatment with CHK1 siRNA caused a pronounced decrease in cell growth in the gemcitabine treated cells compared to the vehicle treated cells. Data is representative of three independent experiments.

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