HT-RNAi kinase screening for identification of sensitizers to gemcitabine. HT-RNAi screens were performed on MIA PaCa-2 cells transfected with a siRNA library targeting 572 kinases followed by treatment with either vehicle or 5 nM or 10 nM gemcitabine. Cell viability was assessed and normalized to control wells. (A) Scatterplot of the log2 values of cell viability for gemcitabine plus siRNA treated cells versus vehicle plus siRNA treated cells showed CHK1 as a significant hit. (B) Plot of log2 ratios of gemcitabine/vehicle for each siRNA treated with either 5 nM or 10 nM gemcitabine. (C) Empirical Probability Distribution of log2 ratios of gemcitabine/vehicle (5 nM and 10 nM). Hit areas are highlighted in red. (D) Venn diagram of gene hits from both the 5 nM (highlighted in pink) and 10 nM (highlighted in yellow) gemcitabine synthetic lethal RNAi screen.