Representative images of Matrigel plugs subcutaneously injected at a magnification of ×60; bar = 40 μm. Endothelial cells are identified by lectin staining, that gives a brown reaction product. Different background is due to counterstaining, performed with a standard mixture of hematoxylin and eosin, as described in Methods (A). Quantification of microvessels infiltrating Matrigel plugs (B). * = p < 0.05 vs VEGF15. Error bars show SEM.