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Figure 1 | Journal of Translational Medicine

Figure 1

From: Can urinary exosomes act as treatment response markers in prostate cancer?

Figure 1

Purification of urine-derived exosomes. Healthy donor urine was subjected to exosome purification, and at each step, 10 μl of sample was kept for electrophoretic analysis (4–20% gradient polyacrylamide gel, silver stained) (A), demonstrating effective removal of the principal non-exosomal protein bands such as that at ~80 Kd, and significant enrichment of diverse protein species in the final exosome product (A). Parallel gels were run for immuno-blot analyses, using antibodies against typical exosome proteins as indicated (B). Comparing the sucrose cushion method, with a simpler method of Pisitkun et al, where cell culture media (C) or fresh urine (D) were subject to centrifugation at 17,000 g followed by pelletting at 200,000 g. Exosomes (from sucrose method) and the 200,000 g pellet were normalised for protein differences, and 2.5 μg/well analysed by western blot for markers as indicated.

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