Figure 1

A: Agarose gel electrophoresis of the PAX2 exon 10 RT-PCR products from the mRNA of the lymphoma cell lines. All lymphoma cell lines: band of 339 bp of varying intensity. KM-H2, EHEB, L540 and DG75: band of the expected size of 185 bp. Negative control: water instead of cDNA, positive control: plasmid (pCR2.1-TOPO) coding for the PAX2 exon 10 PCR product. B: Schematic presentation of the sequencing result of the 339 bp PCR product: Detection of the new PAX2 splice variant containing the whole intron 9 sequence and exon 10 sequence C: Expression level of PAX2 intron 9 specific mRNA: The relative amount was expressed as ratio marker [pg/μl]/PBGD [pg/μl]). The sample concentration was calculated using the plasmid standard curve. Thick bar: median expression level. D: Analysis of the expression of the different PAX2 splice variants by Western Blot: The known splice variants of 43–46 kDa and the new splice variant of 37 kDa are exemplarily shown for the colon carcinoma cell line HCT116 and lymphoma cell line KM-H2.