Figure 2
From: CTLA4 blockade increases Th17 cells in patients with metastatic melanoma
IL-17 quantification by ELISA. A and B) Pre- and post-dosing IL-17 cytokine determined in culture supernatants of whole PBMC (A) or CD4+-sorted cells (B) after stimulation for 4 days with anti-CD3 and anti-CD28. The supernatant was collected for IL-17 quantitation using an ELISA assay (p values by pairwise t-test). C) Multicytokine array in the same ex vivo stimulated samples quantifying IL-1β, IL-2, IL-4, IL-5, IL-10, IL-12(p70), IL-13, TNFα, and RANTES.