Inhibition of MAP kinases activation after PGSE treatment. U937 cells were treated with PGSE (1 or 3 mg/ml) for 24 hours followed by 20 units/ml TNF-α stimulation for 2 hours. Whole cell protein lysate was analyzed by Western analysis using (A) anti-phospho ERK1/2 antibodies; and (B) anti-phospho p38MAPK antibodies as described in Methods. (C) Cell lysate with PGSE treatment only was analyzed by anti-phospho ERK1/2 antibodies. Equal amount of protein loading in the blot was shown by staining the immunoblot with anti-ERK1/2 or anti-p38MAPK antibodies. PGSE, 70% ethanol-water extracts of ginseng.