Figure 4

(A) The mean ± SD hexanaldehyde concentration in the bioreactor headspace of media and HL60 cells are presented at 4-h (empty bar), 24-h (gray bar) and 48-h (black bar) of incubation. Headspace hexanaldehyde concentration is significantly higher from HL60 cells compared to media (p < 0.0001). Significantly different levels of hexanaldehyde are emitted at 24-h and 48-h incubations compared to 4-h from HL60 cells (4-h 1.1 ± 0.3 ppbv, 24-h 8.1 ± 1.7 ppbv and 48-h 10.8 ± 2.2 ppbv). * represents concentrations significantly higher compared to 4-h from HL60 cells, and # represents significant higher hexanaldehyde generation from HL60 cells compared to media. (B) Representative chromatograms of hexanaldeyde after 48 hours of incubation. The low VOC air was used to flush the headspace of the bioreactors containing vials of media and HL60 prior to incubation. An equal volume of air was analyzed in each of the three chromatograms.