Skip to main content

Table 1 Scoring methods based on p16INK4a expression patterns and subcellular loci in ECA and EMA

From: Scoring mechanisms of p16INK4a immunohistochemistry based on either independent nucleic stain or mixed cytoplasmic with nucleic expression can significantly signal to distinguish between endocervical and endometrial adenocarcinomas in a tissue microarray study

Scoring Method

Score categories

ECA

EMA

p-value

C

Score 0–3

9 (64.3%)

20 (83.3%)

.245a

 

Score 4–12

5 (35.7%)

4 (16.7%)

 
 

Median (Range)

2 (0–12)

2 (0–12)

.152

N

Score 0–3

3 (21.4%)

17 (70.8%)

.006a

 

Score 4–12

11 (78.6%)

7 (29.2%)

 
 

Median (Range)

5 (2–12)

2 (0–9)

<0.001b

Mean of C plus N

Score 0–3

4 (28.6%)

21 (87.5%)

<0.001a

 

Score 4–12

10 (71.4%)

3 (12.5%)

 
 

Median (Range)

4.25 (2–12)

2 (0–10.5)

<0.001b

  1. PS:
  2. 1. C: Method of scoring based on independent cytoplasmic staining alone, irrespective of nucleic staining.
  3. 2. N: Method of scoring based on independent nucleic staining alone, irrespective cytoplasmic staining.
  4. 3. Mean of C plus N: Method of scoring based on mean of cytoplasmic score plus nucleic score.
  5. Note:
  6. • The "a" is Chi-squared test with continuity correction or Fisher exact test, the "b" is Mann-Whitney U test using exact significant.
  7. •Using score 4 points as a cutoff, the immunostains are defined "negative" for scores from 0 to 3, and "positive" for scores from 4 to 12 points.