Figure 4

(A) – Co-localization of RES and CAV1 in HepG2 cells – Transport of dansyl chloride-derived RES with green fluorescence (A) and recombinant CAV1 distribution with red fluorescence (B) and the two combination of both (C). Pooled HepG2 cells and CAVM2 cells bearing green (D) or red fluorescence (E). Over-expression of CAV1 by CAVM2 cell groups separates transfected from nontransfected HepG2 cells (E); the images are over-imposed in (F). Experiments were repeated 3 times, with similar results. (B) – Immunohistochemistry of tissue microarrays. (Magnification 400×). Immunoreactivity of CAV1 (red) and topoisomerase-alpha (Buffy) in HepG2 variant xenografts with (RES+) or without RES treatment (RES-). (C) Immunofluorescence of CAV1 and topoisomerase-alpha – Comparison of untreated (left) or RES-treated for 24 h (100 μM) (right). Localization of CAV1 and topoisomerase-alpha was visualized by indirect immunofluorescence. Microphotographs of a single field stained with anti-CAV1 (green) and topoisomerase-alpha (red) antibodies. (Magnification 400×) Experiments were repeated 3 times, with similar results.