Figure 7
From: MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells
Measurement of differentially expressed genes byqRT-PCR. Quantitative real-time PCR confirmed the expression of 3 genes found by microarray analysis to be upregulated in hES: POU5F1 (OCT4), LEFTY1, and TDGF1, and 2 genes upregulated in EB: HAND1 and GATA5, and 1 gene upregulated in adult cells: NFIB. In addition, the levels of another hES cell marker Nanog was also measured. The results were normalized by endogenous control 18s rRNA and the fold change was calculated by equation 2-ΔΔCt. The y-axis indicates the Log2-transformed fold change relative to the calibrator.