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Figure 9 | Journal of Translational Medicine

Figure 9

From: Generation in vivo of peptide-specific cytotoxic T cells and presence of regulatory T cells during vaccination with hTERT (class I and II) peptide-pulsed DCs

Figure 9

A. Cytotoxicity against peptide-pulsed T2 cells before and after 2 cycles of vaccination (N001–N010, phase I). Enhanced cytotoxicity before and after 2 cycles of vaccination with peptide-labelled T2 cells. Graph on the left shows cytotoxicity of PBMCs generated using DCT vaccine, that on the right shows cytotoxicity of PBMCs generated using DCTI vaccine; 10,000 PKH-labelled T2 events were acquired and analysed. Statistically significant cytotoxicity was observed following 2 vaccinations compared with baseline (X: p = 0.04, Wilcoxon signed rank test). Values are represented as median (bar), interquartile range (box) and range (whiskers). B. Cytotoxicity against peptide-pulsed T2 cells before and after 2 cycles of vaccination (L001–L006, phase II): Cumulative cytotoxicity (mean, SD) before and after 2 cycles of vaccination with peptide labelled T2 cells at an effector to target cell ratio of 10:1. Unlabelled T2 cells were used as a negative control; 10,000 PKH-labelled T2 events were acquired and analysed. Statistically significant cytotoxicity was observed following 2 vaccinations compared with baseline (X: p = 0.028, Wilcoxon signed rank test). Values are represented as median (bar), interquartile range (box) and range (whiskers).C. SCC-4 targeted ex vivo cytotoxicity of PBMCs (N001–N007, phase I): Cumulative cytotoxicity of T cells generated by 3 re-stimulations of naïve patient PBMCs in vitro by DCT & DCTI with p540, p865 or no peptide; 10,000 SCC-4 cells were incubated with 100,000 PBMCs for this assay.10,000 PKH+SCC-4 events were acquired and analysed. Results are represented as mean (SD). Statistically significant SCC-4 cytotoxicity was observed with DCT and DCTI re-stimulated PBMCs compared with PBMCs restimulated without the class I peptides (X: p < 0.001, t-test), thus showing evidence of cytotoxicity against naturally processed hTERT peptides of SCC-4.

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