Figure 6

Tetramer+ CD8+T cell responses (mean +/- SD) to class I ± II hTERT pulsed DCs. Vaccinations with DCTs pulsed with class I hTERT epitope alone compared with or without class II epitopes in 6 patients. DCTs pulsed with class I+II epitopes showed a strong tendency to enhance tetramer+CD8+T cell responses which were significant (p < 0.05, t-test) at specific vaccination time points V2, V3, V4 and V6 (ie 4 out of the 6 post-vaccination time points). CD8+T cell tetramer+ response to an irrelevant HLA*A201 MAGE antigen, not used in the vaccination, was measured as a negative control; 150,000 events were acquired and analysed.