Figure 8

Assessment of apoptotic cell death. CD437, BA and LND induce apoptosis of ADF cells. (A) An apoptotic nucleus. (B) Percentage of apoptotic cells (assuming as 100% the number of total cells) in drug treated samples and in vehicle treated controls 24 and 48 hours after treatment. The number of apoptotic cells counted in the drug treated samples was compared with that of the vehicle treated controls using the unpaired t-test. *P < 0.05; **P < 0.001. (C) A representative example of annexin V binding assay. Several cells show annexine V cross reactivity on their membranes in CD437, BA and LND treated samples. In the merged panel Annexin V and PI signals appear purple and cyan respectively.