Figure 2

Differential PCR. ADF cells did not show EGFR amplification and have a hemizygous deletion of the chromosome 10q region containing the PTEN locus. Amplification bands of representative experiments are depicted for each gene in the analized samples. NB1, normal brain tissue sample 1; NB2, normal brain tissue sample 2; GBIV, WHO grade IV glioblastoma; E/G, mean ratio of densitometry values of EGFR and GAPDH amplification bands. P/G, mean ratio of densitometry values of PTEN and GAPDH amplification bands.