Expression of p53, p21/WAF1 and MDM2 in HCC by Western blot. The expression of p53, p21/WAF1, and MDM2 was detected in hepatocellular carcinoma (HCC) tissues by western blot analysis. We used 7 pairs of HCC tissues and the adjacent hepatic tissues. Tissues T1-7 were HCC tissues and N1-7 were the adjacent hepatic tissues. The expression of the housekeeping gene, glyceraldehydes 3-phosphate dehydrogenase (GAPDH), served as a control. The expression of p53 was higher in the HCC tissues (T1-7) than in the adjacent hepatic tissues (N1-7). The MDM2 expression followed a similar trend in both the tissues. The expression of p21/WAF1 was higher in HCC tissues (T1-3, T5-7) than the adjacent hepatic tissues (N1-3, N5-7).