Table 1 Differentiation and maturation procedures used in the present study.
Dendritic cell differentiation | ||
IL-4 differentiated dendritic cells(IL-4 DCs) | ||
GM-CSF | 800 IU/mL | Gentaur, Brussels, Belgium |
IL-4 | 20 ng/mL | R&D Systems, Minneapolis, USA |
IL-15 differentiated dendritic cells(IL-15 DCs) | ||
GM-CSF | 800 IU/mL | Gentaur, Brussels, Belgium |
IL-15 | 200 ng/mL | Immunotools, Friesoythe, Germany |
Dendritic cell maturation | ||
cc-mDC(conventional maturation cocktail, according to Jonuleit et al. [34]) | ||
TNF-α | 10 ng/mL | Biosource, Nivelles, Belgium |
IL-1β | 10 ng/mL | R&D Systems, Minneapolis, USA |
IL-6 | 15 ng/mL | Biosource, Nivelles, Belgium |
PGE2 | 1 μg/mL | Pfizer, Puurs, Belgium |
TLR-mDC(TLR7/8 agonist-based maturation cocktail) | ||
R-848 (Resiquimod) | 3 μg/mL | Alexis Biochemicals, San Diego, USA |
TNF-α | 2.5 ng/mL | Biosource, Nivelles, Belgium |
IFN-γ | 5000 IU/mL | Immunotools, Friesoythe, Germany |
PGE2 | 1 μg/mL | Pfizer, Puurs, Belgium |