Figure 1

Phenotypic characteristics of immature IL-15 DCs. Immature DCs were analyzed by flow cytometry for the expression of CD1a, CD14, CD56, CD80, CD207 (Langerin) and CD209 (DC-SIGN). The histograms represent the expression of the indicated cell surface antigens (bold-line histograms) and the corresponding isotype controls (grey-filled histograms). The mean ± SEM percentage of positive cells (%) and delta MFI ± SEM (ΔMFI) were calculated as specified in the "Methods" section (n = 3-6). (a) Phenotype of monocyte-derived DCs generated in the presence of GM-CSF + IL-15 and harvested at the immature stage 2-3 days after initiation of the DC culture. (b) Corresponding phenotypic profile of conventional immature DCs, differentiated in the presence of GM-CSF + IL-4.