Doxil treated ovarian cancer cells upregulate MHC class-I and Fas expression in vitro. (A) ID8 cells were exposed to titered concentrations of Doxil (0, 0.3, 1 and 3 ug/ml) and measured for viable cell countsmeasured. ID8 cells were either incubated in culture media alone or with the indicated concentration of Doxil for 6 hours, washed, and incubated in drug-free media for 42 hours before harvesting. (B) Upregulation of MHC-I (left) and Fas (right) on ID8 cells following treatment with Doxil and staining with MHC-I and Fas antibodies. Histogram: Isotype control (red); untreated (blue); Doxil 0.1 μg/ml (green); Doxil 1 μg/ml (brown). All the histograms depict Annexin-V negative (non apoptotic cells). B) Dot plot diagrams depict the upregulation of MHC-I and Fas in gated non-apoptotic (Annexin v-negative) tumor cells exposed to Doxil 42 hours before.