Figure 5

CpG induced maturation of transduced pDC. Comparative phenotype of transduced GEN2.2, 6 days posttransduction, in the absence and presence of CpG for 24 hours. Overlay histograms show the expression of relevant antigens for transduced GEN2.2 cultured without CpG (thin line), with CpG (thick line) and with CpG and gated on E-GFP⁺ (green line) versus isotype-matched controls (dotted line). (A, B, C) Transduced GEN2.2 vectors are the same ones as those described in figure 2. Values indicated are MFI of the transduced populations cultured without CpG versus transduced populations cultured with CpG. The results are representative of at least 4 experiments.