Immunophenotype of transduced pDC. Comparative phenotypes of transduced and untransduced GEN2.2 in absence of maturation agent, at day 5. Overlay histograms show the expression of CD123 or HLA-DR for untransduced (thin line), total transduced (thick line) and E-GFP+ gated (green line) GEN2.2, versus isotype-matched controls (dotted line). (A) GEN2.2 transduced by LV with a PGK promoter pseudotyped with either VSVG, RD114 or GaLV envelopes. (B) GEN2.2 were transduced with VSVG pseudotyped-LV with a PGK, EF1, desmin or C5–12 promoter (C) GEN2.2 were transduced by rAAV of serotype 1, 2 or 5 with a CMV promoter. The results are representative of at least 4 experiments.