Figure 3From: Highly efficient transduction of human plasmacytoid dendritic cells without phenotypic and functional maturationImmunophenotype of transduced pDC. Comparative phenotypes of transduced and untransduced GEN2.2 in absence of maturation agent, at day 5. Overlay histograms show the expression of CD123 or HLA-DR for untransduced (thin line), total transduced (thick line) and E-GFP+ gated (green line) GEN2.2, versus isotype-matched controls (dotted line). (A) GEN2.2 transduced by LV with a PGK promoter pseudotyped with either VSVG, RD114 or GaLV envelopes. (B) GEN2.2 were transduced with VSVG pseudotyped-LV with a PGK, EF1, desmin or C5–12 promoter (C) GEN2.2 were transduced by rAAV of serotype 1, 2 or 5 with a CMV promoter. The results are representative of at least 4 experiments.Back to article page