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Figure 3 | Journal of Translational Medicine

Figure 3

From: Comparative study on the immunogenicity between an HLA-A24-restricted cytotoxic T-cell epitope derived from survivin and that from its splice variant survivin-2B in oral cancer patients

Figure 3

HLA-A24-binding assay of peptides. Binding affinity of peptide to HLA-A24 molecule was evaluated by mean fluorescent intensity (MFI) shift of cell surface HLA-A24 level on T2-A24 cells that were pulsed with each peptide. CMV pp65-derived HLA-A24-binding peptide (QVDPVAALF) and HIV env-derived HLA-A24-binding peptide (RYLRDQQLLGI) were used as positive controls. VSV-derived peptide VSV8 (RGYVYQGL) was used as a negative control. Histograms of MFI shift were displayed for each peptide. MFI shift was calculated as; MFI shift = (MFI of T2-A24 cells pulsed with the peptide) - (MFI of T2-A24 cells without peptide pulsation).

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Journal of Translational Medicine

ISSN: 1479-5876

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