Inhibition of Aurora kinases results in cell death. Cells were treated for 96 hours with differing doses of VE-465. (A) PTX10 cells (B) 1A9 cells. Following treatment cells were harvested, fixed and stained with propidium iodide before analysis by Flow Cytometry. The sub G0/G1 population represents apoptotic cells. Each time point represents data from at least 3 independent experiments. Caspase 3/7 assays of PTX10 (C) and 1A9 (D) cells treated with increasing doses of VE-465 demonstrate dose-dependent increase in apoptosis. The caspase activity was blocked by the pan-caspase inhibitor Z-VAD.