Figure 5
Robustness of assay: comparison of AF488-MCP-1 internalization inhibition at 1 hour and 24 hours. The AF488-MCP-1 internalization assay was tested in whole blood after one hour incubation with a serial dilution of CCR2 inhibitor and compared to incubation with the inhibitor for 24 hours. The IC50 for 1 hour was determined to be 0.9 ± 0.3 μg/ml compared to 1.6 ± 0.6 μg/ml (n = 4). Inhibition of percent positive cells shown with similar results observed for MESF.