Robustness of assay over 3 days. The AF488-MCP-1 internalization assay was tested in whole blood over a 3 day period. CD14+ monocytes and CD4+ memory T cells were examined and the Mean Equivalence of Soluble Fluorescence (MESF) of the CD14+ cells (A) or percentage of CD14+ cells (B) or CD4+ (C) staining positive cells were examined. The percent CV of the parameters over the three day period (n = 5) are 15.5,8.8 and 18.6 respectively. Day 1 represents the initial baseline comparative value.