Skip to main content
Figure 2 | Journal of Translational Medicine

Figure 2

From: Validation of a flow cytometry based chemokine internalization assay for use in evaluating the pharmacodynamic response to a receptor antagonist

Figure 2

Titration of assay reagents. A) AF488-MCP-1 was serially diluted in whole blood and allowed to react at room temperature. CD14+ monocytes were examined and the Mean Equivalence of soluble fluorescence (MESF) was reported. Maximum saturation was determined to be 60–75 nM. B) Titration of CCR2 antagonist against optimum concentration (60 nM) of AF488 MCP-1

Back to article page

Journal of Translational Medicine

ISSN: 1479-5876

Contact us