Figure 1

Immunofluorescence staining of β-tubulin, glial fibrillary acidic protein (GFAP) and galactocerebroside (GalC) in neurosphere-derived cells cultured on poly-L-ornithine- and laminin-coated coverslips displaying a microglial morphology. A: Cells with BrdU-labeled nuclei (green fluorescence) expressing β-tubulin (red fluorescence). B: Cells with propidium iodide-counterstained nucleus (red fluorescence) expressing GFAP (green fluorescence) and C: GalC (green fluorescence)-expressing cell counterstained with Hoechst 333442 (blue fluorescence).