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Table 8 ELISPOT assay normal distribution

From: Validation of a HLA-A2 tetramer flow cytometric method, IFNgamma real time RT-PCR, and IFNgamma ELISPOT for detection of immunologic response to gp100 and MelanA/MART-1 in melanoma patients

 

gp100209

gp100pool

MART-1

Flu

HIV

1

2

3

4

15

2

2

4

2

4

5

1

3

5

4

4

3

2

4

2

2

3

3

2

5

2

2

1

3

1

6

9

5

6

8

7

7

2

2

4

5

4

8

0

1

2

241

1

Mean

3.3

2.6

3.5

35.4

2.5

SD

2.8

1.3

1.5

83.2

2.1

Mean + 2 SD

8.9

5.2

6.5

201.8

6.7

  1. HLA-A2 PBMC (105 cells/well) from healthy donors were stimulated with peptides and the number of IFNγ secreting cells determined.
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Journal of Translational Medicine

ISSN: 1479-5876

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