TIL | TIL1520 | TIL1235 |
---|
TIL specificity | gp100209 | MART-1 |
---|
Peptide specificity | Relevant | Relevant | Irrelevant | Relevant | Irrelevant | Irrelevant |
---|
Peptide | gp100209 | gp100pool | MART-1 | MART-1 | gp100209 | gp100pool |
---|
1 | 44 | 41 | 1 | 8 | 2 | 2 |
2 | 42 | 31 | 1 | 9 | 0 | 1 |
3 | 37 | 40 | 1 | 10 | 2 | 1 |
4 | 43 | 32 | 1 | 10 | 1 | 1 |
5 | 38 | 52 | 2 | 7 | 1 | 1 |
Mean (n = 5) | 41 | 39 | 1.1 | 9 | 1.3 | 1.3 |
SD | 3.1 | 8.5 | 0.4 | 1.2 | 0.8 | 0.6 |
- TIL cells (80 cells/well) were spiked in two different lots of PBMC (105 cells/well), stimulated with peptides, and analyzed for the number of IFNγ secreting cells per well (average value from triplicate wells). Two analysts each performed five assays. The numbers of IFNγ secreting cells from 2 PBMC lots by two analysts were found to be comparable. Data (secreting cells, Mean from 5 tests, and SD) from PBMC lot 1 by analyst 1 is shown as an example.