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Table 1 Tetramer assay precision

From: Validation of a HLA-A2 tetramer flow cytometric method, IFNgamma real time RT-PCR, and IFNgamma ELISPOT for detection of immunologic response to gp100 and MelanA/MART-1 in melanoma patients

 

Tetramer

gp100

MART-1

 

Cells

TIL1520

TIL1143

Intra assay

   

High

Range

54.48–57.21

3.33–3.96

 

Mean (n = 5)

56.15

3.64

 

SD

1.14

0.23

 

%CV

2

6

Low 1

Individual Value

1.05, 1.32

 
 

Mean (n = 2)

1.19

 
 

SD

0.19

 
 

%CV

16

 

Low 2

Individual Value

0.52, 0.60

 
 

Mean (n = 2)

0.56

 
 

SD

0.06

 
 

%CV

10

 

Inter assay

   

High

Range

41.86–62.63

3.26–4.65

 

Mean (n = 5)

53.38

3.74

 

SD

9.44

0.55

 

%CV

18

15

Long term

   

High

Range

62.69–99.49

1.45–7.75

 

Mean (n = 48)

93.24

4.10

 

SD

6.48

1.85

 

%CV

7

45

  1. Data is shown as % Tetramer positive cells (Range, Mean, n, SD, and %CV). Cells are used as undiluted (High) or diluted in the negative population (Low 1 and Low 2 for TIL1520). Long term assay precision (inter assay) is shown using data collected in 48 tests.