Figure 7

MART-1 tetramer positive cells detected in a melanoma patient upon vaccination. Patient's PBMC was analyzed for % MART-1 tetramer positive cells. Cells stained with negative control tetramer (upper panel) and MART-1 tetramer (lower panel) is shown. Tetramer positive cells in dot blots on gated viable lymphocytes are shown. No binding is seen among CD8 negative cell population. Percent tetramer positive cells shown are calculated based on gated viable CD8⁺ T cells. Trend analysis demonstrated statistical significant linear response.